Luteinizing Hormone (LH)
Product Specification:
Luteinizing hormone is a gonadotropin and a glycoprotein having two subunits. The alpha subunit is similar to those of FSH, HCG, and TSH. The beta subunit is different from the other hormones and confers its biochemical specificity. LH is secreted by the basophilic cells of the anterior pituitary. LH is involved in the maturation of the ovarian follicles, ovulation and the formation of the corpus luteum. LH is available as whole molecule standard (50%) and High purity (95%) grades, or as alpha and beta subunits.
General Information:
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Molecular Weight: 28,000 Daltons alpha- 13,500 beta- 14,500
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Source: Human Pituitary Extract
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IU/mg Standard grade 5,000 IU/mg
-
IU/mg High purity 8,500/mg
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Form: lyophilized powder
Storage - 20 degrees C
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Stability: 5 years from date of manufacture
Method of Analysis:
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Purity is determined by SDS-Page.
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Acceptable level Standard grade: 50%
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Acceptable level High purity: 95%
Activity is determined by one of the following methods:
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Method DPC Immulite 1000
Reference Preparation WHO 1st IRP (68/40) and 2nd IS 80/552 -
Method ACS 180
Reference Preparation WHO 2nd IRP (80/552)
Contaminants Tested:
|
Contaminant |
Method |
Acceptable level; Standard Grade |
Acceptable level; High Purity |
|
FSH |
ACS 180 |
Less than 0.5% |
Less than 0.2% |
|
TSH |
ACS 180 |
Less than 2% |
Less than 0.2% |
|
PRL |
ACS 180 |
N/A |
Less than 0.1% |
|
GH |
DPC Immulite 1000 |
N/A |
Less than 0.1% |
